A hybrid genome assembly of the endangered aye-aye (Daubentonia madagascariensis)

濒临灭绝的指猴(Daubentonia madagascariensis)的杂交基因组组装

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作者:Cyril J Versoza, Susanne P Pfeifer

Abstract

The aye-aye (Daubentonia madagascariensis) is the only extant member of the Daubentoniidae primate family. Although several reference genomes exist for this endangered strepsirrhine primate, the predominant usage of short-read sequencing has resulted in limited assembly contiguity and completeness, and no protein-coding gene annotations have yet been released. Here, we present a novel, fully annotated, chromosome-level hybrid de novo assembly for the species based on a combination of Oxford Nanopore Technologies long reads and Illumina short reads and scaffolded using genome-wide chromatin interaction data-a community resource that will improve future conservation efforts as well as primate comparative analyses.

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