Abstract
Nowadays, the exploration of natural materials for the production of nanoparticles is of special interest due to its ecofriendly nature. In this paper, we presented the biosynthesis of gold nanoparticles (AuNPs) in a green route by using water extract of pollen from Andean honeybees. Furthermore, AuNPs have been characterized by various techniques and tested for the catalytic reduction of 4-nitrophenol (4-NP). The biosynthesized AuNPs were analyzed using UV-vis spectroscopy, Transmission electron microscopy (TEM), Dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) spectroscopy to confirm their optical properties, stability, surface morphology, and purity. The synthesized AuNPs proved to be well dispersed, spherical, and triangular in shape, with particle sizes ranging from 7 to 42 nm having λmax at 530 nm. Moreover, FTIR suggests the capping of AuNPs with pollen constituents and XRD confirms the crystalline structure of AuNPs. Additionally, prepared AuNPs were demonstrated to be effective in reducing organic pollutant 4-NP to 4-aminophenol (k = 59.17898 × 10-3 min-1, R2 = 0.994). All of these studies have emphasized that AuNPs production can be scale up by using naturally available pollen grains and open up a new perspective for beekeepers.