Isolation of mouse ovarian follicles for single-cell RNA-seq and in vitro culture

Here, we provide a protocol combing single-cell genomics and functional experiments to study stepwise folliculogenesis. This protocol isolates different developmental stages of mouse ovarian follicles to perform single-cell RNA-seq and in vitro culture, allowing to dissect and validate key molecular events in guiding folliculogenesis, thus bringing non-growing oocytes into maturity. For complete details on the use and execution of this protocol, please refer to Gu et al. (2019) and Long et al. (2022).