Conclusions
This equipment is suited to investigators who have an established small animal NMR and wish to explore the potential of heteronuclear ((13)C and (15)N) MRI, MRS, which harnesses the enormous sensitivity gain offered by hyperpolarization.
Methods
Drawing on an earlier, but unpublished, prototype, we provide diagrams of a delivery circuit, a laminar-flow reaction chamber within a low field NMR contained in a compact, movable housing. Assembly instructions are provided from which a computer driven, semi-automated PASADENA polarizer can be constructed.
Results
Together with an available parahydrogen generator, the polarizer, which can be operated by a single investigator, completes one cycle of hyperpolarization each 52 s. Evidence of efficacy is presented. In contrast to competing, commercially available devices for dynamic nuclear polarization which characteristically require 90 min per cycle, PASADENA provides a low-cost alternative for high throughput. Conclusions: This equipment is suited to investigators who have an established small animal NMR and wish to explore the potential of heteronuclear ((13)C and (15)N) MRI, MRS, which harnesses the enormous sensitivity gain offered by hyperpolarization.