Performance characteristics of updated INNO-LiPA assays for molecular typing of human leukocyte antigen A (HLA-A), HLA-B, and HLA-DQB1 alleles

用于人类白细胞抗原 A (HLA-A)、HLA-B 和 HLA-DQB1 等位基因分子分型的最新 INNO-LiPA 检测的性能特征

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作者:Karen De Vreese, Rachel Barylski, Fiona Pughe, Miriam Bläser, Colin Evans, John Norton, Gilbert Semana, Richard Holman, Pascale Loiseau, Dominique Masson, Mieke Gielis, Annelies De Brauwer, Ilse De Canck, Gonda Verpooten, Frank Hulstaert

Abstract

We carried out a multicenter performance evaluation of three new DNA-based human leukocyte antigen (HLA) typing assays: INNO-LiPA HLA-A Update, INNO-LiPA HLA-B Update, and INNO-LiPA HLA-DQB1 Update. After optimization, the accuracy rates were all 100%, and the final observed resolutions were 99.4, 92.4, and 85.6%, respectively. These rapid and easy-to-perform assays yielded results fully concordant with other DNA-based tissue typing tests.

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