Abstract
Current skin sensors or wound dressings fall short in addressing the complexities and challenges encountered in real-world scenarios, lacking adequate capability to facilitate wound repair. The advancement of methodologies enabling early diagnosis, real-time monitoring, and active regulation of drug delivery for timely comprehensive treatment holds paramount significance for complex chronic wounds. In this study, a nanocomposite hydrogel is devised for real-time monitoring of wound condition and comprehensive treatment. Tannins and siRNA containing matrix metalloproteinase-9 gene siRNA interference are self-assembled to construct a degradable nanogel and modified with bovine serum albumin. The nanogel and pH indicator are encapsulated within a dual-crosslinking hydrogel synthesized with norbornene dianhydride-modified paramylon. The hydrogel exhibited excellent shape adaptability due to borate bonding, and the click polymerization reaction led to rapid in situ curing of the hydrogel. The system not only monitors pH, temperature, wound exudate alterations, and peristalsis during wound healing but also exhibits hemostatic, antimicrobial, anti-inflammatory, and antioxidant properties, modulates macrophage polarization, and facilitates vascular tissue regeneration. This therapeutic approach, which integrates the monitoring of pathological parameters with comprehensive treatment, is anticipated to address the clinical issues and challenges associated with chronic diabetic wounds and infected wounds, offering broad prospects for application.