Abstract
Protein kinase C (PKC) plays critical roles in neuronal activity and is widely expressed in striatal neurons. However, it is not clear how PKC activation regulates the excitability of striatal cholinergic interneurons. In the present study, we found that PKC activation significantly inhibited A-type potassium current (I(A)), but had no effect on delayed rectifier potassium currents. Consistently, application of PKC activator caused an increase of firing in response to depolarizing currents in cholinergic interneurons, which was persistent in the presence of both excitatory and inhibitory neurotransmission blockers. These excitatory effects of PKC could be partially mimicked and occluded by blockade of I(A) with potassium channel blocker 4-aminopyridine. In addition, immunostaining demonstrated that PKCalpha, but not PKCgamma and PKCepsilon, was expressed in cholinergic interneurons. Furthermore, activation of group I metabotropic glutamate receptors (mGluRs) led to an inhibition of I(A) through a PKC-dependent pathway. These data indicate that activation of PKC, most likely PKCalpha, increases the neuronal excitability of striatal cholinergic interneurons by down-regulating I(A). Group I mGluR-mediated I(A) inhibition might be important for the glutamatergic regulation of cholinergic tone in the neostriatum.