Immunogenicity of chimeric MUC1-HER2 vaccine against breast cancer in mice

小鼠抗乳腺癌嵌合MUC1-HER2疫苗的免疫原性

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作者:Elaheh Gheybi, Ali Hatef Salmanian, Abbas Ali Imani Fooladi, Jafar Salimian, Hamideh Mahmoodzadeh Hosseini, Raheleh Halabian, Jafar Amani

Conclusion

The results implied a critical role of HER2 and MUC1 antibodies in vaccination against breast cancer. This engineered protein can be a good vaccine to stop breast cancer.

Methods

BALB/c mice were immunized with this novel chimeric protein. The humoral immune response was assessed by enzyme-linked immunosorbent assay (ELISA). Then, BALB/c mice were injected subcutaneously 2×105 4T1-MUC1-HER2 tumor cells. Subsequently, tumor size and tumor necrosis measurements, MTT, cytokines assay and survival test were performed.

Results

The results implied a critical role of HER2 and MUC1 antibodies in vaccination against breast cancer. This engineered protein can be a good vaccine to stop breast cancer.

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