Conclusion
Our findings indicate that scaffolds with 3D structure and osteoinductive growth factors have great potential in bone tissue engineering.
Methods
In this study, core-shell poly(lactide-co-glycolide) (PLGA)/polycaprolactone (PCL)-BMP-2 (PP-B) fibrous scaffolds were prepared through coaxial electrospinning. Next, we fabricated 3D scaffolds based on PP-B fibers with thermally induced self-agglomeration (TISA) method and compared with conventional PLGA/PCL scaffolds in terms of scaffold morphology and BMP-2 release behaviors. Then, rat adipose-derived stem cells (rADSCs) were seeded on the scaffolds, and the effects on cell proliferation, cell morphology, and osteogenic differentiation of rADSCs were detected.
Results
The results demonstrated that 3D scaffold incorporated with BMP-2 significantly increased proliferation and osteogenic differentiation of rADSCs, followed by PP-B group.