Conclusion
Using UHDs increases the production of photosynthetic pigments the root length and the number of lateral roots. Also, the amount of protein, gibberellic acid and abscisic acid in seedlings treated using native UHDs of C. officinalis (native or commercial) is higher than the others.
Methods
High-performance thin-layer chromatography (HPTLC) is used to isolate the active compounds. On the separated
Purpose
This article deals with producing ultra-diluted compounds (UHDs) prepared from Iranian calendula's characteristic and endemic species. It compares their chemical, biological and biochemical characteristics with the commercial sample of calendula species (grown in the Alps). In the following, these UHDs have been used to improve the quality of germination and growth and reduce contamination of rice (Oryza sativa) seeds in the laboratory environment.
Results
The HPTLC reveals that the chromatogram of native C. percica and C. officinalis extract is the most similar to the commercial compounds. The highest antioxidant activity is related to C. officinalis. The best antibacterial activity of the extracts against Staphylococcus aureus and Escherichia coli belongs to C. officinalis and C. tripterocarpa. Rutin, quercitrin, β-campstrole and di-o-caffeoylquinic acid, which are among the flavonoid and terpenoid categories were identified as active compounds. The prepared UHDs from native calendula are biologically more effective than the commercial ones in increasing seed germination efficiency, improving rooting quality and reducing contamination.