Abstract
Cardenolides, a group of cardiac glycosides are potent inhibitors of Na+/K+ ATPase pump in mammals, animals including insects. Some insects can circumvent the toxicity of cardenolides by mechanisms like target site resistance and metabolic resistance resulting in enhanced tolerance or adaptation. In this paper, we report an intriguing observation of a polyphagous feeder feeding gregariously on the leaves of Calotropis gigantea (L.) without any apparent adverse effect. No choice feeding assay showed higher larval biomass and reduced number of days to develop on C. gigantea leaves compared to Ricinus and banana. We found the activity of GST higher in C. gigantea fed larva and HR LC-MS analysis of Olepa sps. revealed the presence of glutathione-strophanthidin conjugate in larval body tissue. In silico molecular simulation results confirmed strong interaction between delta variant GST and glutathione-strophanthidin complex. The sequestration site and cost benefit of glutathione-strophanthidin sequestration in body tissues of Olepa sps. needs further investigation.