Development of microsatellite loci in Scrophularia incisa (Scrophulariaceae) and cross-amplification in congeneric species

玄参科(Scrophulariaceae)中微卫星位点的开发及其在同属物种中的交叉扩增

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作者:Rui-Hong Wang, Chuan Chen, Qing Ma, Pan Li, Cheng-Xin Fu

Conclusions

These results indicate that these microsatellite markers are adequate for detecting and characterizing population genetic structure in the Chinese species of sect. Tomiophyllum at fine and range-wide geographical scales.

Results

Using the compound microsatellite marker technique, 12 microsatellite primers were identified in S. incisa. The number of alleles ranged from 14 to 26 when assessed in 78 individuals from four populations. With high cross-species transferability, these primers also amplified in S. dentata and S. kiriloviana. • Conclusions: These results indicate that these microsatellite markers are adequate for detecting and characterizing population genetic structure in the Chinese species of sect. Tomiophyllum at fine and range-wide geographical scales.

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