Characterization of 10 new nuclear microsatellite markers in Acca sellowiana (Myrtaceae)

Acca sellowiana(桃金娘科)中 10 个新核微卫星标记的表征

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作者:Gustavo H F Klabunde, Denise Olkoski, Vinicius Vilperte, Maria I Zucchi, Rubens O Nodari

Conclusions

These new polymorphic SSR markers will allow future genetic studies to be denser, either for genetic structure characterization of natural populations or for studies involving genetic breeding and domestication process in A. sellowiana.

Results

A total of 10 polymorphic microsatellite primers were designed from clones obtained from a simple sequence repeat (SSR)-enriched genomic library. The primers amplified di- and trinucleotide repeats with four to 27 alleles per locus. In all tested populations, the observed heterozygosity ranged from 0.269 to 1.0. • Conclusions: These new polymorphic SSR markers will allow future genetic studies to be denser, either for genetic structure characterization of natural populations or for studies involving genetic breeding and domestication process in A. sellowiana.

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