Conclusions
Improved understanding of the connection between HLA-ABC, HLA-DR, and bacterial extracts such as RA10 may lead to the development of drug design and therapies related to breast cancer condition in which these receptors are involved.
Methods
The expression of HLA-ABC and HLA-DR was assessed by flow cytometry and confocal microscope on the human breast tumor cell lines MDA-MB-231, which used a readily accessible model system. It is postulated that the HLA-ABC and HLA-DR receptors might be regulated by bacillus bacterial extracts designated (RA10, RA4, RA7, and RA16).
Objective
The expression of human leukocyte antigen (HLA)-ABC and HLA-DR is linked to the development of breast cancer. This study was aimed to determine the effect of bacillus bacterial extracts on the expression of the major histocompatibility complexes (MHC) Class I and Class II receptors on breast cancer cells.
Results
It was observed that the treatment of cell line MDA-MB-231 with the RA10 resulted in an upregulation of the cell surface expression of the HLA-A, B, C receptors. It was observed that RA10 has the capacity to reduce the expression of HLA-A, B, C, it also shows a detectable degree of cytotoxicity when used at high concentrations. The data show that the cell surface expression of HLA-ABC is higher than HLA-DR. No significant changes of HLA-DR expression were observed on MDA-MB-231 cell lines. Conclusions: Improved understanding of the connection between HLA-ABC, HLA-DR, and bacterial extracts such as RA10 may lead to the development of drug design and therapies related to breast cancer condition in which these receptors are involved.