The draft genomes of Crassostrea gasar and Crassostrea rhizophorae: key resources for leveraging oyster cultivation in the Southwest Atlantic

加沙牡蛎 (Crassostrea gasar) 和红牡蛎 (Crassostrea rhizophorae) 基因组草图:促进西南大西洋牡蛎养殖的关键资源

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作者:Nicholas Costa Barroso Lima, Luiz Gonzaga Paula de Almeida, Afonso Celso Dias Bainy, Alexandra Lehmkuhl Gerber, Ana Paula de Campos Guimarães, Antonio Mateo Solé-Cava, Claudio Manoel Rodrigues de Melo, Cristiano Lazoski, Flávia Lucena Zacchi, Frederico Henning, Leticia Maria Monteiro Soares, Rafaela

Abstract

Objectives: The two oyster species studied hold considerable economic importance for artisanal harvest (Crassostrea rhizophorae) and aquaculture (Crassostrea gasar). Their draft genomes will play an important role in the application of genomic methods such as RNAseq, population-based genomic scans aiming at addressing expression responses to pollution stress, adaptation to salinity and temperature variation, and will also permit investigating the genetic bases and enable marker-assisted selection of economically important traits like shell and mantle coloration and resistance to temperature and disease. Data description: The draft assembly size of Crassostrea gasar is 506 Mbp, and of Crassostrea rhizophorae is 584 Mbp with scaffolds N50 of 11,3 Mbp and 4,9 Mbp, respectively. The general masked bases by RepeatMasker in both genomes were highly similar using different datasets. The masked bases varied from 9.41% in C. gasar to 10.05% in C. rhizophorae and 42.85% in C. gasar to 44.44% in C. rhizophorae using Dfam and RepeatModeler datasets, respectively. Functional annotation with eggNog resulted in 34,693 annotated proteins in C. rhizophorae and 26,328 in C. gasar. BUSCO analysis shows that almost 99% of genes (5,295) are complete in relation to the mollusk orthologous genes dataset (mollusca_odb10).

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