Crystallization and crystallographic analysis of the apo form of the orange protein (ORP) from Desulfovibrio gigas

巨型脱硫弧菌橙蛋白 (ORP) 的载脂蛋白形式的结晶和晶体学分析

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作者:Shabir Najmudin, Cecília Bonifácio, Américo G Duarte, Sofia R Pauleta, Isabel Moura, José J G Moura, Maria J Romão

Abstract

The orange-coloured protein (ORP) from Desulfovibrio gigas is a 12 kDa protein that contains a novel mixed-metal sulfide cluster of the type [S(2)MoS(2)CuS(2)MoS(2)]. Diffracting crystals of the apo form of ORP have been obtained. Data have been collected for the apo form of ORP to 2.25 A resolution in-house and to beyond 2.0 A resolution at ESRF, Grenoble. The crystals belonged to a trigonal space group, with unit-cell parameters a = 43, b = 43, c = 106 A.

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