Culture Separation, Identification and Unique Anti-pathogenic Fungi Capacity of Endophytic Fungi from Gucheng Salvia Miltiorrhiza

古城丹参内生真菌的培养分离、鉴定及独特抑菌能力

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作者:Guo-Kai Wang, Jai-Sing Yang, Yu-Fei Huang, Jin-Song Liu, Chia-Wen Tsai, DA-Tian Bau #, Wen-Shin Chang #

Background

Salvia miltiorrhiza is a medical herb for human disorders including cardiovascular diseases and cancer. However, the interactions between Salvia miltiorrhiza and its endophytes are largely unknown. The current study aimed at identifying its endophytic fungi and examining their inhibitory effects on anti-pathogenic fungus. Materials and

Conclusion

The anti-fungal activities of the endophytic fungus from Salvia miltiorrhiza were confirmed for the first time, and this may benefit crop quality and production in the future.

Methods

Distinct species of endophytic fungi were isolated from the roots of Salvia miltiorrhiza, cultured, sequenced, aiming to predict their taxonomical structures. Meanwhile, extracts from each endophytic fungus fermentations were isolated, compared and evaluated on the inhibitory efficacies on five pathological fungi, Cercospora nicotianae, Phoma arachnidicola, Staphylococcus, Phytophthora eggplant, and Rhizoctonia cerealis.

Results

A total of 34 strains of endophytic fungi were obtained from Salvia miltiorrhiza. Among them, SX19 and C. Gloeosporioids exhibited the most effective inhibitions on five pathogenic fungi.

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