Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols

用于严重急性呼吸综合征冠状病毒 2 分子检测的增强型快速商业 DNA 提取试剂盒:轻松适应当前协议

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作者:Rômulo Pessoa-E-Silva, Priscilla Stela Santana de Oliveira, Sayonara Maria Calado Gonçalves, Klarissa Miranda Guarines, Lidiane Vasconcelos do Nascimento Carvalho, Maria Andreza Bezerra Correia, Michelle Melgarejo da Rosa, Moacyr Jesus Barreto de Melo Rêgo, Maira Galdino da Rocha Pitta, Michelly Cri

Conclusions

The low cost and rapidity of the enhanced protocol makes it suitable for adoption in laboratories diagnosing COVID-19, especially those with high demand for examinations.

Methods

After testing several different modifications, the adapted protocol with the best

Results

Processing and testing of 119 nasopharyngeal samples ultimately yielded near-perfect agreement with the automated protocol (κ = 0.981 [95% confidence interval 0.943-1.000]). Conclusions: The low cost and rapidity of the enhanced protocol makes it suitable for adoption in laboratories diagnosing COVID-19, especially those with high demand for examinations.

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