Characterisation of New Foxunavirus Phage Murka with the Potential of Xanthomonas campestris pv. campestris Control

新型 Foxunavirus 噬菌体 Murka 的表征及其对 Xanthomonas campestris pv. campestris 的控制潜力

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作者:Peter V Evseev, Rashit I Tarakanov, Ha T N Vo, Natalia E Suzina, Anna A Vasilyeva, Alexander N Ignatov, Konstantin A Miroshnikov, Fevzi S-U Dzhalilov

Abstract

Phages of phytopathogenic bacteria are considered to be promising agents for the biological control of bacterial diseases in plants. This paper reports on the isolation and characterisation of a new Xanthomonas campestris pv. campestris phage, Murka. Phage morphology and basic kinetic characteristics of the infection were determined, and a phylogenomic analysis was performed. The phage was able to lyse a reasonably broad range (64%, 9 of the 14 of the Xanthomonas campestris pv. campestris strains used in the study) of circulating strains of the cabbage black rot pathogen. This lytic myovirus has a DNA genome of 44,044 bp and contains 83 predicted genes. Taxonomically, it belongs to the genus Foxunavirus. This bacteriophage is promising for use as a possible means of biological control of cabbage black rot.

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