Abstract
The certification of death due to mechanical asphyxia has been a complex problem in some cases. The use of protein expression to identify mechanical asphyxia death has recently attracted attention. Asphyxia creates an extremely hypoxic environment for cells, which should reactivate the mitochondria in the cells. Cyto c and AIF, located in the mitochondria, are transferred to the cytoplasm under hypoxia to trigger the apoptotic process. Based this phenomenon, we designed the animal asphyxia model and cell hypoxia model to examine whether Cyto c and AIF are expressed in the cytoplasm, and we used human samples to verify the results. We found that the two proteins were detectably expressed in the cytoplasm of mechanical asphyxia groups and were hardly detected in the cytoplasm of other groups. This is a promising finding that may shed light on the precise mechanisms associated with mechanical asphyxia.