Abstract
An experiment was conducted to investigate the effect of inoculum volume (IV), substrate quantity, and the use of a manual or automated gas production (GP) recording system for in vitro determinations of fermentation of corn distillers dried grains with solubles (cDDGS) and soybean hulls (SBH). A 2 × 2 × 2 factorial arrangement of treatments was used and included the factors of (1) ingredients (cDDGS or SBH), (2) inoculum volume and substrate quantity (IV30 = 0.2 g substrate + 30 mL inoculum or IV75 = 0.5 g substrate + 75 mL inoculum), and (3) GP recording system (MRS = manual recording system or ARS = automated recording system). Feed ingredient samples were pre-treated with pepsin and pancreatin, and the hydrolyzed residues were subsequently incubated with fresh pig feces in a buffered mineral solution. The GP recording was monitored for 72 h, and the kinetics were estimated by fitting data using an exponential model. Compared with SBH, cDDGS yielded less (p < 0.01) maximal gas production (Gf), required more time (p < 0.02) to achieve half gas accumulation (T/2), and had less (p < 0.01) fractional rate of degradation (µ) and in vitro fermentability of dry matter (IVDMF). Using the ARS resulted in less IVDMF (p < 0.01) compared with MRS (79.0% vs. 81.2%, respectively). Interactions were observed between GP recording system and inoculum volume and substrate quantity for Gf (p < 0.04), µ (p < 0.01), and T/2 (p < 0.04) which implies that increasing inoculum volume and substrate quantity resulted in decreased Gf (332 mL/g from IV30 vs. 256 mL/g from IV75), µ (0.05 from IV30 vs. 0.04 from IV75), and T/2 (34 h for IV30 vs. 25 h for IV75) when recorded with ARS but not MRS. However, the recorded cumulative GP at 72 h was not influenced by the inoculum volume nor recording system. The precision of Gf (as measured by the coefficient of variation of Gf) tended to increase for IV30 compared with IV75 (p < 0.10), indicating that using larger inoculum volume and substrate quantity (IV75) reduced within batch variation in GP kinetics. Consequently, both systems showed comparable results in GP kinetics, but considering convenience and achievement of consistency, 75 mL of inoculum volume with 0.5 g substrate is recommended for ARS.