Protein-ligand binding affinity determination by the waterLOGSY method: An optimised approach considering ligand rebinding
通过 waterLOGSY 方法测定蛋白质-配体结合亲和力：一种考虑配体重新结合的优化方法
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作者：Renjie Huang, Arnaud Bonnichon, Timothy D W Claridge, Ivanhoe K H Leung
Abstract
WaterLOGSY is a popular ligand-observed NMR technique to screen for protein-ligand interactions, yet when applied to measure dissociation constants (KD) through ligand titration, the results were found to be strongly dependent on sample conditions. Herein, we show that accurate KDs can be obtained by waterLOGSY with optimised experimental setup.
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