Molecular Biology for Diagnosis of Congenital and Neonatal Infections in the Cerebrospinal Fluid of Newborns from a Brazilian Tertiary Hospital

巴西三级医院新生儿脑脊液中先天性和新生儿感染的分子生物学诊断

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作者:Suzana Ferreira Zimmerman, Rodrigo Gonçalves de Lima, André Moreno Morcillo, Sandra Helena Alves Bonon, Sergio Tadeu Martins Marba

Conclusions

The pathogens identified by PCR were mostly Zika virus, VZV, and B-19, and these were mainly found in newborns with malformations.

Methods

This was a prospective descriptive study involving the molecular analysis of 151 CSF samples from neonates, collected for cytological and biochemical diagnosis from 2017 to 2021. After the

Results

A total of 151 CSF samples were obtained, 16 of which were positive (10.6% [95% CI%: 6.18-16.63%]). Two of these were PCR-positive for HSV-1 (1.3%), four for VZV (2.6%), one for CMV (0.67%), two for Toxoplasmosis (1.3%), four for Parvovirus B-19 (2.6%), and four for Zika (2.6%). The proportion of positive PCR results was higher in the group that presented with malformations (25.0% vs. 8.4%, p = 0.040). Conclusions: The pathogens identified by PCR were mostly Zika virus, VZV, and B-19, and these were mainly found in newborns with malformations.

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