Simplified Method for Kinetic and Thermodynamic Screening of Cardiotonic Steroids through the K(+)-Dependent Phosphatase Activity of Na(+)/K(+)-ATPase with Chromogenic pNPP Substrate

利用生色底物pNPP，通过Na(+)/K(+)-ATPase的K(+)依赖性磷酸酶活性，对强心甾类药物进行动力学和热力学筛选的简化方法