Chemical synthesis of 2″OMeNAD+ and its deployment as an RNA 2'-phosphotransferase (Tpt1) 'poison' that traps the enzyme on its abortive RNA-2'-PO4-(ADP-2″OMe-ribose) reaction intermediate

2″OMeNAD+ 的化学合成及其作为 RNA 2'-磷酸转移酶 (Tpt1)“毒物”的应用，可将该酶捕获在其终止的 RNA-2'-PO4-(ADP-2″OMe-核糖) 反应中间体上。