CRISPR/Cas with ribonucleoprotein complexes and transiently selected telomere vectors allows highly efficient marker-free and multiple genome editing in Botrytis cinerea
利用核糖核蛋白复合物和瞬时选择的端粒载体,CRISPR/Cas技术可对灰葡萄孢菌进行高效的无标记多重基因组编辑。
期刊:PLoS Pathogens
影响因子:5.5
doi:10.1371/journal.ppat.1008326
Thomas Leisen,Fabian Bietz,Janina Werner,Alex Wegner,Ulrich Schaffrath,David Scheuring,Felix Willmund,Andreas Mosbach,Gabriel Scalliet,Matthias Hahn