Precipitation with polyethylene glycol followed by washing and pelleting by ultracentrifugation enriches extracellular vesicles from tissue culture supernatants in small and large scales
用聚乙二醇沉淀,然后通过超速离心进行洗涤和沉淀,可以小规模和大规模地从组织培养上清液中富集细胞外囊泡
期刊:Journal of Extracellular Vesicles
影响因子:15.5
doi:10.1080/20013078.2018.1528109
Anna-Kristin Ludwig, Kyra De Miroschedji, Thorsten R Doeppner, Verena Börger, Johannes Ruesing, Vera Rebmann, Stephan Durst, Sören Jansen, Michel Bremer, Elmar Behrmann, Bernhard B Singer, Holger Jastrow, Jan Dominik Kuhlmann, Fouzi El Magraoui, Helmut E Meyer, Dirk M Hermann, Bertram Opalka, Stefan