日期:
2020 年 — 2026 年
2020
2021
2022
2023
2024
2025
2026
影响因子:

Loss of a newly discovered microRNA in Chinese hamster ovary cells leads to upregulation of N-glycolylneuraminic acid sialylation on monoclonal antibodies

中国仓鼠卵巢细胞中新发现的 microRNA 的丢失导致单克隆抗体上 N-乙醇酰神经氨酰唾液酸化的上调

Simon Fischer, Sven Mathias, Anna Stadermann, Shumin Yang, Valerie Schmieder, Nikolas Zeh, Nicoletta Schmidt, Patrick Richter, Sara Wright, Eike Zimmermann, Yan Ley, Julia van der Meer, Thomas Hartsch, Christian Bernloehr, Kerstin Otte, Harald Bradl, Martin Gamer, Patrick Schulz

Rational optimization of a monoclonal antibody improves the aggregation propensity and enhances the CMC properties along the entire pharmaceutical process chain

单克隆抗体的合理优化可改善聚集倾向并增强整个制药工艺链中的 CMC 特性

Joschka Bauer, Sven Mathias, Sebastian Kube, Kerstin Otte, Patrick Garidel, Martin Gamer, Michaela Blech, Simon Fischer, Anne R Karow-Zwick

Unraveling what makes a monoclonal antibody difficult-to-express: From intracellular accumulation to incomplete folding and degradation via ERAD

揭示单克隆抗体难以表达的原因:从细胞内积累到不完全折叠和通过 ERAD 降解

Sven Mathias, Anna Wippermann, Nadja Raab, Nikolas Zeh, René Handrick, Ingo Gorr, Patrick Schulz, Simon Fischer, Martin Gamer, Kerstin Otte

Visualisation of intracellular production bottlenecks in suspension-adapted CHO cells producing complex biopharmaceuticals using fluorescence microscopy

使用荧光显微镜可视化悬浮适应型 CHO 细胞生产复杂生物制药时的细胞内生产瓶颈

Sven Mathias, Simon Fischer, René Handrick, Jürgen Fieder, Patrick Schulz, Harald Bradl, Ingo Gorr, Martin Gamer, Kerstin Otte

miRNA engineering of CHO cells facilitates production of difficult-to-express proteins and increases success in cell line development

CHO 细胞的 miRNA 工程有助于生产难以表达的蛋白质并提高细胞系开发的成功率

Simon Fischer, Kim F Marquart, Lisa A Pieper, Juergen Fieder, Martin Gamer, Ingo Gorr, Patrick Schulz, Harald Bradl