The complex ATP-Fe(2+) serves as a specific affinity cleavage reagent in ATP-Mg(2+) sites of Na,K-ATPase: altered ligation of Fe(2+) (Mg(2+)) ions accompanies the E(1)-->E(2) conformational change.

ATP-Fe(2+)复合物作为Na,K-ATPase的ATP-Mg(2+)位点的特异性亲和力裂解试剂：Fe(2+) (Mg(2+))离子的配位改变伴随着E(1)-->E(2)构象变化