Combination of the somatic cell nuclear transfer method and RNAi technology for the production of a prion gene-knockdown calf using plasmid vectors harboring the U6 or tRNA promoter

结合体细胞核移植法和RNAi技术，利用含有U6或tRNA启动子的质粒载体，生产朊病毒基因敲低小牛。