Fluorogenic, Subsingle-Turnover Monitoring of Enzymatic Reactions Involving NAD(P)H Provides a Generalized Platform for Directed Ultrahigh-Throughput Evolution of Biocatalysts in Microdroplets
利用荧光法对涉及 NAD(P)H 的酶促反应进行亚单周转监测,为微滴中生物催化剂的定向超高通量进化提供了一个通用平台。
期刊:Journal of the American Chemical Society
影响因子:15.6
doi:10.1021/jacs.4c11804
Penner, Matthew; Klein, Oskar James; Gantz, Maximilian; Nintzel, Friederike E H; Prowald, Anne-Cathrin; Boss, Sally; Barker, Paul; Dupree, Paul; Hollfelder, Florian