I-PREFR: Inverse PCR-Based Restriction Enzyme FRee Unidirectional Strategy for Rapid Markerless Chromosomal Gene Deletion and Reconstitution in Bacteria Using Suicide Vectors.

I-PREFR：基于反向 PCR 的无限制性酶单向策略，利用自杀载体在细菌中快速无标记染色体基因删除和重建