Selection for Tn10 tet repressor binding to tet operator in Escherichia coli: isolation of temperature-sensitive mutants and combinatorial mutagenesis in the DNA binding motif

在大肠杆菌中筛选与 tet 操纵子结合的 Tn10 tet 阻遏蛋白：分离温度敏感突变体和 DNA 结合基序的组合诱变