Anti-Caspase-3 antibody [E87] (Ab32351)

CPP32, CASP3, Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CPP-32, SCA-1

反应种属:Human
抗原名称:CASP3
应用:WB, IHC, FCM, IF/ICC, IP

用户验证的应用

推荐稀释比例

  • IHC-P1/25 - 1/100 Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
  • WB1/5000
  • ICC/IF1 µg/mL For unpurified, use 1/25 dilution.
  • Flow Cyt (Intra)1/180 - 1/1000 ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • IP1/10 - 1/50

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Anti-Caspase-3 antibody [E87]
产品形式Liquid
储存缓冲液pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein A

背景信息

Anti-Caspase-3 antibody [E87] (ab32351) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB in human samples. Anti-Caspase-3 antibody [E87] (ab32351) has been cited over 386 times in peer reviewed journals and is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-Caspase-3 antibody [E87] (ab32351) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-Caspase-3 antibody [E87] (ab32351) has been confirmed by testing in knockout samples. Anti-Caspase-3 antibody [E87] (ab32351) has 11 independent reviews from customers. Anti-Caspase-3 antibody [E87] (ab32351) specifically detects Caspase-3 (UniProt ID: P42574; Molecular weight: 17kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone E87 - ab197202. Antibody clone E87 is also available pre-conjugated to a variety of labels for your convenience - Biotin, PE, APC, HRP, Alkaline Phosphatase, Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 594 ( ab195905, ab305665, ab305666, ab305667, ab308706, ab309678, ab310040, ab310425 ). Species reactivity Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

文献引用 (587)

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一种新型的FGFR3靶向抗体-药物偶联物通过cGAS-STING通路诱导膀胱癌细胞凋亡。

Spatially selective clearance of inflammation-mediated reactive oxygen species in injured kidney cells.

空间选择性清除受损肾细胞中炎症介导的活性氧。

Xie Maowei, Fu Xin, Lan Yufei, Zhang Yuping, Li Haixin, Su Yan, Zhou Kun, Zhang Ying, Ding Xuejing, He Jian, Mao Zhilei

Lactate-driven ATP6V1B2 lactylation triggers asthmatic inflammation by linking lysosomal dysfunction to mitochondrial ROS-dependent pyroptosis.

乳酸驱动的 ATP6V1B2 乳酸化通过将溶酶体功能障碍与线粒体 ROS 依赖性细胞焦亡联系起来,从而引发哮喘炎症。

Aptamer-functionalized exosomes combined with doxorubicin suppress GBM progression and enhance chemoradiosensitivity by promoting pyroptosis.

适体功能化的外泌体与阿霉素联合使用,通过促进细胞焦亡抑制胶质母细胞瘤进展并增强化疗和放射敏感性。

Wang Jing, Peng Li, Zhong Xinxin, Zheng Siqi, Liang Xiuhua, Du Menghan, Xia Xiangning, Guo Ting, Zheng Lidan, Fang Yingying, Tian Yinghong, Shi Yusheng, Zhang Xingmei

Unfolded Protein Response as a Therapeutic Target: 4-(Heptyloxy)phenol Induces Programmed Cell Death in Ewing Sarcoma.

未折叠蛋白反应作为治疗靶点:4-(庚氧基)苯酚诱导尤文氏肉瘤细胞程序性死亡。

Zhang Nenggang, Perez Mary, Jenkins Gaye N, Horton Terzah M, Gilbertson Scott R, Pati Debananda

Omega-3 Fatty Acids Attenuate LPS-Induced Acute Kidney Injury via Activation of AMPK/SIRT1/PGC-1α/NRF2/FOXO3 Signaling and Suppression of NF-κB-Mediated Inflammation.

Omega-3 脂肪酸通过激活 AMPK/SIRT1/PGC-1α/NRF2/FOXO3 信号通路和抑制 NF-κB 介导的炎症来减轻 LPS 诱导的急性肾损伤。

Ahmet Bindal, Halil Asci, Oznur Kolay, Yusuf Tepebasi Muhammet, Sinan Kizilkaya, Ilter Ilhan, Gulsum Bindal Tozlu, Ozlem Ozmen

Interleukin-17A Orchestrates Lung Injury and Remodeling Through p53 and uPA System Crosstalk.

白细胞介素-17A通过p53和uPA系统相互作用来调控肺损伤和重塑。

Das Durgesh Nandini, Balnadupete Akarsha, Shetty Rashmi, Gopu Venkadesa Perumal, Sajjan Rushil, Bhandary Yashodhar P, Marudamuthu Amarnath S, Oliver Christian, Patel Aarav, Patel Aryan, Samten Buka, Iwakura Yoichiro, Tang Hua, Citrin Deborah E, Peters Jay, Shetty Sreerama

Leptin Regulates Intervertebral Disc Calcification and Ossification by Promoting Glycolysis Through OCN/HIF-1α Axis.

瘦素通过 OCN/HIF-1α 轴促进糖酵解,从而调节椎间盘钙化和骨化。

Li Haoxi, Yu Chengqiang, Li Zhuhai, Yao Shuyu, Mo Kaiqi, Peng Guanlu, Huang Yufeng, Wei Jianxun

Cycloastragenol Inhibits Colorectal Cancer Cell Metastasis via Epithelial-Mesenchymal Transition and the PI3K Signalling Pathway.

环阿斯特拉醇通过上皮-间质转化和PI3K信号通路抑制结直肠癌细胞转移。

Miao JingRong, Feng PanFeng

Synergistic effects through targeting the PI3K and IGFR pathways in treating lung cancer carrying activation alterations along the PI3K pathway.

通过靶向 PI3K 和 IGFR 通路发挥协同作用,治疗 PI3K 通路激活改变的肺癌。

Abd El-Salam Mohamed, Chen Wu, Tang Yan, Rao Ting, Kang Xuejia, Sun Lifang, Han Tiegang, Chen Pengyu, Mossanen Matthew, Cheng Fan, Yang Chun, Pan Chong-Xian
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