Anti-NF-kB p65 antibody [E379] (Ab32536)

推荐稀释比例

• IHC-P1/5000 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
• WB1/1000 - 1/100000 This antibody might not detect target band in mouse tissues.
• ICC/IF1/100 For unpurified use at 1/250 -1/500.
• IP1/30 For unpurified use at 1/200. We do not guarantee IP for mouse.

Abcam recommended secondaries - Goat Anti-Rabbit HRP ( ab205718 ) and Goat Anti-Rabbit Alexa Fluor® 488 ( ab150077 ). Or search our wide range of secondary antibodies for use with your experiment. Product Specifications Anti-NF-kB p65 antibody [E379] (ab32536) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, IP, WB in human, mouse samples. Anti-NF-kB p65 antibody [E379] (ab32536) specifically detects NF-kB p65 (UniProt ID: Q04206; Molecular weight: 60kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Quality and Validation Abcam's high quality manufacturing and validation processes ensure Anti-NF-kB p65 antibody [E379] (ab32536) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-NF-kB p65 antibody [E379] (ab32536) has been confirmed by testing in knockout samples. Anti-NF-kB p65 antibody [E379] (ab32536) has been cited over 439 times in peer reviewed journals and is trusted by the scientific community. Anti-NF-kB p65 antibody [E379] (ab32536) has 15 independent reviews from customers. Related Products Antibody clone E379 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Biotin, PE, Alexa Fluor® 594 ( ab190205, ab190589, ab205823, ab208750, ab214846 ). Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies. Species reactivity Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.