Anti-beta Catenin antibody [E247] - ChIP Grade (Ab32572)

CTNNB, OK/SW-cl.35, PRO2286, CTNNB1, Catenin beta-1, Beta-catenin

反应种属:Human, Mouse, Rat
抗原名称:CTNNB1
应用:WB, IHC, IF/ICC, MIHC, IP, ChIP, CUT&RUN-seq

用户验证的应用

推荐稀释比例

  • IHC-P1/500 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • mIHC1/250
  • WB1/5000 - 1/10000 We recommend Goat anti-Rabbit IgG H&L (IRDye ® 800CW) preadsorbed ( ab216773 ).
  • ICC/IF1/250 We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488) preadsorbed ( ab150081 ) secondary antibody.
  • IP1/30

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Anti-beta Catenin antibody [E247] - ChIP Grade
产品形式Liquid
储存缓冲液pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein A

背景信息

Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIP, ICC/IF, IHC-P, IP and WB. Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) was first used in a scientific publication in 1999 and has been cited over 1076 times in peer reviewed journals. It's performance in Western Blot in human, mouse and rat samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) has been confirmed by Western Blot testing in beta Catenin knockout HepG2 cells ( ab277911 ). Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) has 75 independent reviews from customers. Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) specifically detects beta Catenin (UniProt ID: P35222; Molecular weight: 86kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone E247 - ab196204. Antibody clone E247 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, Alexa Fluor ® 647, HRP, Alexa Fluor ® 594, Alexa Fluor ® 555 ( ab194118, ab194119, ab194120, ab201771, ab202496 ). Top cited antibody on the market with >1370 citations. E247 is top rated with >75 reviews. Beta-catenin plays a crucial role in cell adhesion and gene transcription and its dysregulation is linked to various cancers and diseases including, myocardial infarction, arrhythmias, cardiomyopathie and Alzheimers disease Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

文献引用 (1179)

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NONO-HOXA1-Wnt轴在心肌细胞分化中起着至关重要的作用。

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S100B induces angiogenesis via the clathrin/FOXO1/β-catenin signaling pathway and contributes to Bevacizumab resistance in epithelial ovarian cancer.

S100B 通过网格蛋白/FOXO1/β-catenin 信号通路诱导血管生成,并导致上皮性卵巢癌对贝伐珠单抗产生耐药性。

Xu Haoya, Li Wenzhi, Yue Huiran, Bai Yang, Li Jun, Lu Xin, Wang Jieyu

Magnetoelectric nanoparticles drive TAF9B(+) T(H)2 cell expansion to alleviate inflammation.

磁电纳米粒子驱动 TAF9B(+) T(H)2 细胞扩增以减轻炎症。

Song Jia, Liu Lulu, Liu Ziqi, Liu Shuo, Zhang Zhang, Liu Xinyu, Heng Boon Chin, Wang Yaojin, Lu Dan, Zhang Xuehui, Deng Xuliang

Phosphodiesterase 1A overexpression contributes to the progression of renal fibrosis.

磷酸二酯酶 1A 过度表达会促进肾纤维化的进展。

Loss of FAT1 drives cyclophosphamide resistance in breast cancer via the Wnt/β-Catenin pathway

FAT1 的缺失通过 Wnt/β-catenin 通路驱动乳腺癌细胞对环磷酰胺产生耐药性。

Lijing Zhong ,Dongyan Cao ,Chen Zheng ,Liyi Zhang ,Yuxuan Xu ,Shasha Zhao ,Xiao Liu ,Guiying Wei ,Gengming Niu ,Heming Xu ,Xuan Tang ,Jingyuan Wen ,Paul W R Harris ,Jian Zhang ,Aina He ,Dongxi Xiang

Methyltransferase like 3 promotes thyroid folliculogenesis via coordinating cell differentiation and polarization.

甲基转移酶样3通过协调细胞分化和极化来促进甲状腺滤泡发生。

Jiang Ruoyu, Zhu Qibo, Zhang Zhenlei, He Xiao, Liu Yifan, Kan Ronglin, He Xianghui, Guan Haixia

Pinto bean consumption mitigates colorectal tumorigenesis through reshaping gut microbiota and metabolic signatures.

食用斑豆可通过重塑肠道菌群和代谢特征来减轻结直肠肿瘤的发生。

Gao Tianrui, Liu Ziqi, Zhou Yingxin, Huang Ruiqi, Ye Hui, Liu Xuebo, Wang Jin, Song Mingyue, Huang Yichao, Sun Quancai, Peng Ye

Spectrin coordinates cell shape and signaling essential for epidermal differentiation.

Spectrin 协调细胞形状和信号传导,这对表皮分化至关重要。

Guominkang formula alleviates airway inflammation in HDM-induced asthma mice by regulating Wnt/β-Catenin pathway.

国民康方通过调节 Wnt/β-catenin 通路减轻屋尘螨诱发哮喘小鼠的气道炎症。

Zong Yuhan, Kong Jingwei, Yang Fan, Wang Manting, Wang Ji, Wang Qi

Danggui-Baishao herb pair protects against dextran sulfate sodium-induced colitis by modulating the Wnt/β-catenin pathway.

当归-白骨药组合通过调节 Wnt/β-catenin 通路来预防葡聚糖硫酸钠诱发的结肠炎。

Xu Tian, Hou Wei-Xin, Yang Sun-Ting, Shao Yu-Pei, Wang Jing, Han Tao-Tao, Li Jing-Nan
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