Anti-Cleaved Caspase-3 antibody [E83-77] (Ab32042)

推荐稀释比例

• WB1/500 The pro-caspase-3 is cleaved only when apoptosis event occurs. So, in order to detect active Caspase-3, we strongly suggest to induce your samples into apoptotic pathway.
• ICC/IF1/100 - 1/250

Anti-Cleaved Caspase-3 antibody [E83-77] (ab32042) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF and WB. Anti-Cleaved Caspase-3 antibody [E83-77] (ab32042) was first used in a scientific publication in 1994 and has been cited over 610 times in peer reviewed journals. It's performance in Western Blot in human samplesis trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-Cleaved Caspase-3 antibody [E83-77] (ab32042) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-Cleaved Caspase-3 antibody [E83-77] (ab32042) has been confirmed by Western Blot testing in Cleaved Caspase-3 knockout HAP1 cells. Anti-Cleaved Caspase-3 antibody [E83-77] (ab32042) has 25 independent reviews from customers. Anti-Cleaved Caspase-3 antibody [E83-77] (ab32042) specifically detects Cleaved Caspase-3 (UniProt ID: P42574; Molecular weight: 17kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone E83-77 - ab208003. Cleaved Caspase-3 is a crucial marker of apoptosis, the programmed cell death process. It is the activated form of Caspase-3, resulting from proteolytic cleavage. Cleaved Caspase-3 plays a vital role in executing apoptosis by cleaving various cellular substrates, leading to cell dismantling. Researchers use Cleaved Caspase-3 as a reliable indicator of apoptosis in studies of cancer, neurodegenerative diseases and other conditions involving cell death. Its detection is essential for understanding the mechanisms of apoptosis and developing targeted therapies. In cancer research, cleaved Caspase-3 is used to assess the effectiveness of anti-cancer therapies that induce apoptosis in tumor cells. Elevated levels of cleaved Caspase-3 have been associated with better prognosis in certain cancers, such as oral tongue squamous cell carcinoma. Additionally, cleaved Caspase-3 has been implicated in non-apoptotic roles, such as promoting angiogenesis and chemotherapy resistance. Neurodegenerative Diseases: In neurodegenerative diseases like Alzheimer's (AD) and Parkinson's (PD) disease, cleaved Caspase-3 is involved in the apoptotic pathways that lead to neuronal cell death. For instance, caspase-3-cleaved Tau aggregates more easily than full-length Tau, contributing to the pathology of Alzheimer's disease. Understanding the role of cleaved Caspase-3 in these diseases helps researchers develop targeted therapies to mitigate neuronal loss and disease progression. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies. Species reactivity Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.