Anti-p53 antibody [Y5] (Ab32049)

特异性

This antibody clone recognises both wild-type and mutant forms of p53 in human samples. It is not designed to recognise any specific p53 mutation. We have confirmed this experimentally and have been able to detect p53 in different cell lines using various applications and treatments. Important note: p53 expression levels vary greatly between cell lines. It has been reported that p53 mutations render the protein more stable, hence mutated cell lines often express higher levels of the p53 protein compared to wild-type cell lines. For low expressing wild type cell lines, p53 expression can be increased with cell treatments such as camptothecin or irinotecan.

What is this antibody validated in? Anti-p53 antibody [Y5] (ab32049) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples. What is the molecular weight of p53? Anti-p53 [Y5] (ab32049) specifically detects a band for p53 (UniProt: P04637) at a molecular weight of 44kDa. Trusted by the scientific community Anti-p53 [Y5] (ab32049) was first used in a scientific publication in 2006 and has been cited over 50 times in peer-reviewed journals. Specificity confirmed The specificity of Anti-p53 antibody [Y5] (ab32049) has been confirmed by Western blot testing in TP53 Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [Y5] also available for your convenience: ab32049, HRP - ab190335, Carrier free - ab219731, Alexa Fluor® 488 - ab224920, APC - ab310851, PE - ab310921, Alexa Fluor® 647 - ab311085, Alexa Fluor® 594 - ab311682, Alexa Fluor® 568 - ab312957, Alexa Fluor® 555 - ab313166, Alexa Fluor® 750 - ab321560 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies. Species reactivity Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.