Anti-c-Myc antibody [Y69] - ChIP Grade (Ab32072)

BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39

反应种属:Human, Mouse, Rat
抗原名称:MYC
应用:WB, IHC, FCM, IF/ICC, IP, ChIP-seq, CUT&RUN-seq

用户验证的应用

推荐稀释比例

  • IHC-P5 µg/mL ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
  • WB1/1000
  • ICC/IF5-10 µg/mL 1/100.
  • Flow Cyt (Intra)1/50
  • IP5 µg/mL
  • ChIC/CUT&RUN-seq5 µg
  • ChIP-seq8 µg for 10^7 Cells

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Anti-c-Myc antibody [Y69] - ChIP Grade
产品形式Liquid
储存缓冲液pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein A

背景信息

Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIC/CUT&RUN-seq, ChIP,-seq Flow Cyt (Intra), ICC/IF, IHC-P and IP. Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) was first used in a scientific publication in 1985 and has been cited over 1320 times in peer reviewed journals. It's performance in Western blot and IHC in human and mouse samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) has been confirmed by Western Blot testing in c-Myc knockout HEK-293T cells ( ab256500 ). Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) has 55 independent reviews from customers. c-Myc antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) has been validated in ab256500 ChIC/CUT&RUN-seq, ChIP,-seq Flow Cyt (Intra), ICC/IF, IHC-P and IP. Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072) specifically detects c-Myc (UniProt ID: P01106; Molecular weight: 51kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone Y69 - ab168727. Antibody clone Y69 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, Alexa Fluor ® 647, Alexa Fluor ® 594, Alexa Fluor ® 555, HRP, FITC ( ab190026, ab190560, ab201775, ab201780, ab205818, ab223913 ). Only antibody in the market to this target validated for both CUT&RUN and ChIP-seq, which are key applications to map protein-DNA interactions on a genome-wide scale using NGS. Highly-specific antibody targeting c-Myc, a crucial transcription factor involved in various cellular processes including cancer biology, cell cycle regulation and apoptosis studies. Widely used in cancer research, this antibody is crucial for investigating c-Myc's role in tumorigenesis, including Burkitt lymphoma, multiple myeloma and glioblastoma. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

文献引用 (1453)

Hepatic zonation determines tumorigenic potential of mutant β-catenin.

肝脏分区决定突变β-catenin的致瘤潜力。

Raven Alexander, Gilroy Kathryn, Jin Hu, Waldron Joseph A, Leslie Holly, Munro June, Hall Holly, Ridgway Rachel A, Ford Catriona A, Gulhan Doga C, Vlahov Nikola, Mills Megan L, Hartley Andrew, Anderson Eve, Bryson Sheila, Sphyris Nathalie, Müller Miryam, May Stephanie, Cadden Barbara, Nixon Colin, Waddell Scott H, Guest Rachel, Boulter Luke, Barker Nick, Clevers Hans, Zhu Hao, Ivaska Johanna, Strathdee Douglas, Miller Crispin J, Jamieson Nigel B, Bushell Martin, Park Peter J, Bird Thomas G, Sansom Owen J

Lineage-determining transcription factors constrain cohesin to drive multi-enhancer oncogene regulation.

谱系决定性转录因子限制黏连蛋白,从而驱动多增强子癌基因调控。

Zhou Yeqiao, Jay Atishay, Burget Noah, Friedrich Tobias, Yoon Sora, Alsing Jessica, Nir Guy, Grosschedl Rudolf, Vahedi Golnaz, Faryabi Robert B

MYC modulates TOP2A diffusion to promote substrate detection and activity.

MYC 调节 TOP2A 扩散以促进底物检测和活性。

Cameron Donald P, Jackson Kathryn, Loffreda Alessia, Möller Carl, Kuzin Vladislav, Mazzocca Matteo, Iliopoulou Evanthia, Kolbeinsdottir Hallgerdur, Paluda Andrej, Pavlova Evgeniya, Jagodic Bea, Lopez Duran Brian Saidel, Lamour Valérie, Westerlund Fredrik, Mazza Davide, Baranello Laura

SOX2 confers tumour permissiveness in a specific skin progenitor population.

SOX2 赋予特定皮肤祖细胞群肿瘤易感性。

Centeno Patricia P, Chester Christopher, Kanellos Georgios, Ford Catriona A, Cammareri Patrizia, Inman Gareth J, Jamieson Thomas, Ridgway Rachel A, Marais Richard, Campbell Andrew D, Sansom Owen J

Mosaic partial epidermal reprogramming remodels neighbors and niches to refine skin homeostasis and repair.

表皮部分重塑嵌合体重塑邻近细胞和微环境,以改善皮肤稳态和修复。

Kwak Minjun, Choi Eunjun, Jo Yemin, Kim Boa, Lim Chaeryeong, Lim Jooyoung, Choi Yoon Ha, Lee Jung Hyun, Kim Kee-Pyo, Koo Bon-Kyoung, Kim Jong Kyoung, Choi Sekyu

Heterobifunctional proteomimetic polymers for targeted degradation of MYC and KRAS.

用于靶向降解 MYC 和 KRAS 的异双功能蛋白质模拟聚合物。

Dual targeting of AMRC12 and Malassezia globosa disrupts MYC liquid condensates-driven nuclear pore complex biogenesis in neuroblastoma.

双重靶向 AMRC12 和马拉色菌可破坏神经母细胞瘤中 MYC 液态凝聚物驱动的核孔复合体生物合成。

KDM4A promotes NEPC progression through regulation of MYC expression.

KDM4A 通过调节 MYC 表达促进 NEPC 进展。

Mak Celia Sze Ling, Zhu Ming, Fu Jie, Liang Xin, Wang Xiaoxuan, Yuan Fei, Wang Feng, Hoang Anh G, Song Xingzhi, Shepherd Peter, Liang Derek, Suh Jessica, Contreras Jordan, Dang Thisawin, Yan Cindy, Figueroa Brandon, Mazzocco Mathias, Luo Athena, Pradhan Bijeta, Park Jiwon, Bashir Mirrah, Zhang Miao, Metzger Eric, Schüle Roland, Jain Abhinav K, Karasik Ellen, Frigo Daniel, Foster Barbara A, Lee Min Gyu, Corn Paul, Logothetis Christopher J, Aparicio Ana, Navone Nora, Troncoso Patricia, Tan Zhi, Zhang Jianhua, Lin Sue-Hwa, Wang Guocan

MYC-Mediated Functional Manifestation of IDH1 Mutations in Intrahepatic Cholangiocarcinoma Confers Sensitivity to (+)-JQ1.

MYC介导的IDH1突变在肝内胆管癌中的功能表现赋予了对(+)-JQ1的敏感性。

Wang Fangyanni, Liu Xinyu, Zhang Ning, Kong Ruirui

Loss of UFL1 confers enzalutamide resistance of prostate tumors by regulating METTL16-mediated m6A modification of EEF1A1 mRNA.

UFL1 的缺失通过调节 METTL16 介导的 EEF1A1 mRNA 的 m6A 修饰,使前列腺肿瘤对恩扎卢胺产生耐药性。

Wu Xu, Gao Hui, Zhuang Qianfeng, Li Feng, Feng Xingliang, Shao Naiyuan, Zhang Wei, Zhang Yuyang, Zhang Xiansheng
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