Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (Ab40772)

推荐稀释比例

• IHC-P1/500 - 1/14000 Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
• WB1/1000 - 1/50000 For unpurified protein: 1/200000 dilution
• ICC/IF0.2-1 µg/mL
• Flow Cyt (Intra)1/30 ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For purified format use at 1/1000.

Abcam recommended secondaries - Goat Anti-Rabbit HRP ( ab205718 ) and Goat Anti-Rabbit Alexa Fluor® 488 ( ab150077 ). Or search our wide range of secondary antibodies for use with your experiment. Product Specifications Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt, Flow Cyt (Intra), ICC/IF, IHC-P, WB, mIHC in human samples. Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) specifically detects E Cadherin (UniProt ID: P12830; Molecular weight: 80kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Quality and Validation Abcam's high quality manufacturing and validation processes ensure Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) has been confirmed by testing in knockout samples. Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) has been cited over 750 times in peer reviewed journals and is trusted by the scientific community. Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) has 21 independent reviews from customers. Related Products Antibody clone EP700Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 594, PE, APC ( ab185013, ab194982, ab206878, ab206880, ab224959, ab224960 ). Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies. Species reactivity Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.