Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (Ab68428)

Glial fibrillary acidic protein, GFAP

反应种属:Human, Mouse, Rat
抗原名称:GFAP
应用:WB, IHC, FCM, IF/ICC, MIHC, IP

用户验证的应用

推荐稀释比例

  • IHC-P1/250 - 1/500 Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
  • mIHC1/500 - 1/2000
  • WB1/10000
  • ICC/IF0.1-1 µg/mL
  • Flow Cyt (Intra)1/500
  • IP1/20 - 1/40
  • IHC-Fr1/250 - 1/1000

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
产品形式Liquid
储存缓冲液pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein A

背景信息

Find all reagents to label astrocytes in our " Astrocytes markers guide ". Anti-GFAP antibody [EPR1034Y] (ab68428) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, IP and WB. Anti-GFAP antibody [EPR1034Y] (ab68428) was first used in a scientific publication in 2011 and has been cited over 106 times in peer reviewed journals. It's performance in IHC in mouse samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-GFAP antibody [EPR1034Y] (ab68428) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-GFAP antibody [EPR1034Y] (ab68428) has 15 independent reviews from customers. Anti-GFAP antibody [EPR1034Y] (ab68428) specifically detects GFAP (UniProt ID: P14136; Molecular weight: 50kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone EPR1034Y - ab218309. Antibody clone EPR1034Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, Alexa Fluor ® 647, Alexa Fluor ® 594, Alexa Fluor ® 555, Alexa Fluor ® 568, Alexa Fluor ® 405, PE, APC, HRP, Alkaline Phosphatase, Alexa Fluor ® 647, Biotin, Alexa Fluor ® 488, Alexa Fluor ® 647, Alexa Fluor ® 647, Alexa Fluor ® 488, Alexa Fluor ® 488 ( ab194324, ab194325, ab201732, ab201735, ab201736, ab206586, ab303005, ab303006, ab303007, ab308965, ab313596, ab314264, ab317669, ab317720, ab317721, ab317762, ab319022 ). NEW: Explore our alternative host species for clone [EPR1034Y]: goat, chicken, rat, mouse. GFAP (Glial Fibrillary Acidic Protein) is a key marker in neuro research, particularly for its role in identifying astrocyte activity. It is predominantly expressed in astrocytes, the most abundant glial cells in the brain and spinal cord. Altered GFAP levels are associated with various neurological conditions, including Alzheimer's disease (AD), traumatic brain injury (TBI) and multiple sclerosis (MS). Researchers use GFAP to assess astrocytic response and neuroinflammation, making it essential for understanding the extent of brain injury and the progression of neurodegenerative diseases. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

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评估胶质母细胞瘤中孕激素受体的调节:从体外和动物模型到人体试验方案。

Arcos-Montoya Denisse, García-López Patricia, Wegman-Ostrosky Talia, Camacho-Arroyo Ignacio, Valdés-Rives Silvia Anahí, Bello-Alvarez Claudia, Manjarrez-Marmolejo Joaquín, De La Fuente-Granada Marisol, Ordaz-Ramos Alejandro, Ávila-González Daniela, Díaz Néstor Fabián, Guadarrama-Rangel Carlos Fabricio, Navarrete Andrés Mauricio Bonilla, Aboud Orwa, Cantú-de-León David F, Cacho-Díaz Bernardo, González-Arenas Aliesha

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