Anti-CD31 antibody [EPR3094] (Ab76533)

推荐稀释比例

• IHC-P1/100 - 1/500 We suggest to program the pressure cooker to run for 30 seconds at 125° C, followed by 10 seconds at 90° C. Then let the slide cool down to room temperature (10 - 20 minutes). The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
• WB1/5000 - 1/20000 Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Hu Isoform 1-6: 79-83 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates ( ab7899 ); Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate ( ab7179 ).
• ICC/IF1/500 Use 0.3M glycine to quench autofluorescence caused by aldehydes. Positive Control: HUVEC cells

Anti-CD31 antibody [EPR3094] (ab76533) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, WB in human samples. Anti-CD31 antibody [EPR3094] (ab76533) has been cited over 80 times in peer reviewed journals and is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-CD31 antibody [EPR3094] (ab76533) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-CD31 antibody [EPR3094] (ab76533) has 11 independent reviews from customers. Anti-CD31 antibody [EPR3094] (ab76533) specifically detects CD31 (UniProt ID: P16284; Molecular weight: 80kDa) and is sold in 100 µL and 1 mL selling sizes. Conjugation-ready, carrier free format available for antibody clone EPR3094 - ab207090. Antibody clone EPR3094 is also available pre-conjugated to a variety of labels for your convenience - Biotin, Alexa Fluor® 647, Alexa Fluor® 488, Alexa Fluor® 594, Alexa Fluor® 555 ( ab199734, ab218582, ab275989, ab277231, ab279331 ). This antibody shows no cross-reactivity with rat and mouse samples in WB. However it can give some non specific staining on mouse smooth muscle tissues. Plesae contact our Scientific support for more information. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.