Anti-AKT1 (phospho S473) antibody [EP2109Y] (Ab81283)

特异性

AKT1 (phospho S473) antibody detects AKT1 phosphorylated at Serine 473. The region surrounding S473 in AKT1 is highly similar to the corresponding regions in AKT2 and AKT3, and the immunogen used for this product shares 100% homology with these isoforms. Therefore, cross-reactivity with AKT2 and AKT3 phosphorylated at the equivalent serine residue is possible, although this has not been confirmed experimentally.

Anti-AKT1 (phospho S473) antibody [EP2109Y] (ab81283) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Dot blot, IHC-P, In-Cell ELISA, WB in human, mouse, rat, recombinant fragment samples. Anti-AKT1 (phospho S473) antibody [EP2109Y] (ab81283) has been cited over 612 times in peer reviewed journals and is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-phospho AKT antibody (Anti-RAC antibody; anti-pAKT antibody) [EP2109Y] (ab81283) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-AKT1 (phospho S473) antibody [EP2109Y] (ab81283) has 10 independent reviews from customers. Anti-AKT1 (phospho S473) antibody [EP2109Y] (ab81283) specifically detects AKT1 Phospho-S473 (UniProt ID: P31749; Molecular weight: 56kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone EP2109Y - ab215873. Antibody clone EP2109Y is also available pre-conjugated to a variety of labels for your convenience - HRP, Alexa Fluor® 488, Alexa Fluor® 647 ( ab194201, ab313593, ab313670 ). AKT1 (RAC) also known as protein kinase B (PKB), is a crucial component of the PI3K/AKT signaling pathway, which regulates cell growth, survival, and metabolism. It interacts with key cell cycle proteins like p21, p27, and cyclin D1, and tumor suppressors such as p53. In oncology, AKT1 is a significant target due to its role in promoting tumor growth by enhancing cell proliferation and inhibiting apoptosis. Abcam recommended secondaries - Goat Anti-Rabbit HRP ( ab205718 ) and Goat Anti-Rabbit Alexa Fluor® 488 ( ab150077 ). Or search our wide range of secondary antibodies for use with your experiment. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.