Anti-HMGB1 antibody [EPR3507] (Ab79823)

推荐稀释比例

• IHC-P1/350 - 1/400 Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
• WB1/10000 - 1/50000
• ICC/IF1/250
• Flow Cyt (Intra)1/30 ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Anti-HMGB1 antibody [EPR3507] (ab79823) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB. Anti-HMGB1 antibody [EPR3507] (ab79823) was first used in a scientific publication in 2011 and has been cited over 275 times in peer reviewed journals. It's performance in Western Blot in human, mouse and rat samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-HMGB1 antibody [EPR3507] (ab79823) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-HMGB1 antibody [EPR3507] (ab79823) has been confirmed by Western Blot testing in HMGB1 knockout HeLa cells ( ab255395 ). Anti-HMGB1 antibody [EPR3507] (ab79823) has 7 independent reviews from customers. Anti-HMGB1 antibody [EPR3507] (ab79823) specifically detects HMGB1 (UniProt ID: P09429; Molecular weight: 25kDa) and is sold in 100 µL and 1 mL selling sizes. Conjugation-ready, carrier free format available for antibody clone EPR3507 - ab216986. Antibody clone EPR3507 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, Alexa Fluor ® 647, HRP, Alexa Fluor ® 594, Alexa Fluor ® 405, Alexa Fluor ® 555, APC, PE ( ab195010, ab195011, ab195012, ab206894, ab206895, ab206896, ab225041, ab225042 ). HMGB1 promotes inflammation and tissue damage in diseases like sepsis, cancer and autoimmune disorders by acting as a damage-associated molecular pattern (DAMP) and activating immune responses. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.