Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [4H8] - ChIP Grade (Ab5408)

POLR2, POLR2A, DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, 3'-5' exoribonuclease, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1

反应种属:Human, Mouse, Rat
抗原名称:POLR2A pS5
应用:WB, ELISA, FCM, IF/ICC, ChIP, Dot

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Mouse / IgG1
抗体类型Monoclonal
产品形式Liquid
纯化方式Affinity purification Protein A
储存缓冲液pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Blue Ice 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
无载体蛋白No

背景信息

What is this antibody validated in? Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [4H8] - ChIP Grade (ab5408) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, ChIP, Dot Blot in Human, Mouse, Rat samples. What is the molecular weight of RNA polymerase II CTD repeat YSPTSPS? Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) [4H8] - ChIP Grade (ab5408) specifically detects a band for RNA polymerase II CTD repeat YSPTSPS (UniProt: P24928) at a molecular weight of 217kDa. Trusted by the scientific community Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) [4H8] - ChIP Grade (ab5408) was first used in a scientific publication in 2003 and has been cited over 360 times in peer-reviewed journals. Reviewed by scientists Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) [4H8] - ChIP Grade (ab5408) has over 20 independent reviews from customers. Other related products We have a range of other formats of antibody clone [4H8] also available for your convenience: ab5408, Carrier free - ab264109 The production method has been switched from hybridoma to recombinant on 24th Oct 2025. Want a custom formulation? This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

文献引用 (355)

A single-cell multiomics roadmap of zebrafish spermatogenesis reveals regulatory principles of male germline formation.

斑马鱼精子发生的单细胞多组学路线图揭示了雄性生殖细胞形成的调控原理。

Burgos-Ruiz Ana María, Geng Fan-Suo, Pujol Gala, Sanabria Estefanía, Brethouwer Thirsa, Almuedo-Castillo María, Ruiz-Herrera Aurora, Tena Juan J, Bogdanovic Ozren

Phosphoproteome Remodeling upon CDK1 Inhibition Restricts HSV-1 IE Gene Transcription and Replication.

CDK1抑制引起的磷酸化蛋白质组重塑限制了HSV-1 IE基因的转录和复制。

Rodzkin Maxim S, Honeycutt Drew R, Davido David J

RNA-binding proteins TDP-43 and FUS promote R-loop resolution and regulate transcription termination.

RNA结合蛋白TDP-43和FUS促进R环的解离并调节转录终止。

Zhao Dorothy Yanling, Nabeel-Shah Syed, Ni Zuyao, Pu Shuye, Zhong Guoqing, Schmitges Frank W, Braunschweig Ulrich, Blencowe Benjamin J, Greenblatt Jack F

A prevalent disease-associated SNP in the human ID3 gene regulates E-protein activity and cellular proliferation.

人类 ID3 基因中常见的与疾病相关的 SNP 调节 E 蛋白活性和细胞增殖。

Henderson Christopher A, Ransegnola Brett P, Garmey James, Khan Ali, Aherrahrou Rédouane, Purdy Michael, Wu Shijie, Zhou Andrea, Kirby Jennifer L, Lipinski Michael J, Gordon Vicki, Yeager Mark, Civelek Mete, McNamara Coleen A

Topoisomerase I inhibition suppresses nuclear blebbing via RNA Pol II stalling and nuclear stiffening.

拓扑异构酶 I 抑制剂通过 RNA 聚合酶 II 停滞和核硬化来抑制核膜起泡。

Borowski Nickolas, Li Andy, Stephens Andrew D

SSUP-72/PINN-1 coordinates RNA-polymerase II 3' pausing and developmental gene expression in C. elegans.

SSUP-72/PINN-1 协调秀丽隐杆线虫中的 RNA 聚合酶 II 3' 暂停和发育基因表达

Stubbe François-Xavier, Ponsard Pauline, Steiner Florian A, Hermand Damien

ACSS2-Mediated Histone H4 Lysine 12 Crotonylation (H4K12cr) Alleviates Colitis via Enhancing Transcription of CLDN7.

ACSS2 介导的组蛋白 H4 赖氨酸 12 巴豆酰化 (H4K12cr) 通过增强 CLDN7 的转录来缓解结肠炎

Yuan Ming, Chen Shaopeng, Lin Zhensen, Yu Runfeng, Chao Kang, Ye Shubiao, Li Qing, Ke Haoxian, Zhang Chi, Huang Junfeng, Liang Guanzhan, Hu Tuo, Gao Xiang, Lan Ping, Wu Xianrui

A U3 snoRNA is required for the regulation of chromatin dynamics and antiviral response in Drosophila melanogaster.

U3 snoRNA 是果蝇染色质动力学和抗病毒反应调控所必需的

Jain Shruti, Planells Jordi, Regadas Isabel, Barrett Donal, von Euler Anne, Sinha Indranil, Lindberg Bo Gustav, Hesson Jenny C, Swacha Patrycja, Gekara Nelson O, Pelechano Vicent, Engström Ylva, Mannervik Mattias, Visa Neus

TCF3 activates super-enhancer-driven TRIB2 overexpression to suppress ferroptosis and promote hepatoblastoma proliferation.

TCF3 激活超级增强子驱动的 TRIB2 过表达,从而抑制铁死亡并促进肝母细胞瘤增殖。

Cohesin positions the epigenetic reader Phf2 within the genome

Cohesin 将表观遗传读取器 Phf2 定位在基因组内

Wen Tang #, Lorenzo Costantino #, Roman Stocsits, Gordana Wutz, Rene Ladurner, Otto Hudecz, Karl Mechtler, Jan-Michael Peters
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