Anti-PCNA antibody [EPR3821] (Ab92552)

DNA sliding clamp PCNA, Cyclin, Proliferating cell nuclear antigen, PCNA

反应种属:Human, Mouse, Rat
抗原名称:PCNA
应用:WB, IHC, FCM, IF/ICC, IP

用户验证的应用

推荐稀释比例

  • IHC-P1/100 - 1/1000 The use of an HRP/AP polymerized antibody is recommended for a secondary antibody. Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • WB1/1000 - 1/10000
  • ICC/IF1/100 - 1/250 Use with methanol fixed samples.
  • Flow Cyt (Intra)1/40 For unpurified, use 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • IP1/20 - 1/50

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Anti-PCNA antibody [EPR3821]
产品形式Liquid
储存缓冲液pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Stable for 12 months at -20°C
纯化方式Affinity purification Protein A

背景信息

What is this antibody validated in? Anti-PCNA antibody [EPR3821] (ab92552) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of PCNA? Anti-PCNA [EPR3821] (ab92552) specifically detects a band for PCNA (UniProt: P12004) at a molecular weight of 29kDa. Trusted by the scientific community Anti-PCNA [EPR3821] (ab92552) was first used in a scientific publication in 2010 and has been cited over 420 times in peer-reviewed journals. Reviewed by scientists Anti-PCNA [EPR3821] (ab92552) has over 10 independent reviews from customers. Other related products We have a range of other formats of antibody clone [EPR3821] also available for your convenience: ab92552, Alexa Fluor ® 488 - ab193963, Alexa Fluor ® 647 - ab193964, HRP - ab193965, Carrier free - ab218310, PE - ab314272, ab322083, Carrier free - ab322919 Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

文献引用 (514)

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宁乡猪源粪肠球菌通过乙酸/丙酸-MyD88-NF-β轴保护仔猪免受大肠杆菌引起的肠道菌群失调和炎症。

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具有增强效果的压电支架通过电机械免疫耦合促进骨软骨缺损的愈合。

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靶向 FN1 以克服胆囊癌吉西他滨耐药性:机制见解和 iRGD 修饰的 PEG-PLGA 纳米颗粒递送策略。

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Pinto bean consumption mitigates colorectal tumorigenesis through reshaping gut microbiota and metabolic signatures.

食用斑豆可通过重塑肠道菌群和代谢特征来减轻结直肠肿瘤的发生。

Gao Tianrui, Liu Ziqi, Zhou Yingxin, Huang Ruiqi, Ye Hui, Liu Xuebo, Wang Jin, Song Mingyue, Huang Yichao, Sun Quancai, Peng Ye

Daikenchuto ameliorates dextran sulfate sodium-induced acute and chronic ulcerative colitis by regulating gut microbiota-derived indoles to activate AhR signaling.

大建中汤通过调节肠道菌群衍生的吲哚来激活 AhR 信号,从而改善葡聚糖硫酸钠诱发的急性和慢性溃疡性结肠炎。

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Cycloastragenol Inhibits Colorectal Cancer Cell Metastasis via Epithelial-Mesenchymal Transition and the PI3K Signalling Pathway.

环阿斯特拉醇通过上皮-间质转化和PI3K信号通路抑制结直肠癌细胞转移。

Miao JingRong, Feng PanFeng

Ginsenoside Rg5 inhibits colorectal cancer, at least partially by blocking the lysosomal degradation of colorectal cancer cells.

人参皂苷 Rg5 可抑制结直肠癌,至少部分原因是它阻断了结直肠癌细胞的溶酶体降解。

Guo Xiangwei, Sun Rongyin, Su Yating, Qin Qinghua, Liu Yu, Qiu Chunhong

Aortotomy-induced acute mural thrombosis progresses to saccular aneurysm formation.

主动脉切开术引起的急性壁内血栓形成可发展为囊状动脉瘤。

Bai Hualong, Li Zhuo, Dardik Alan

MiR-641 targets TMEFF2/MEK/PI3K to promote stem cell characteristics of pancreatic cancer cells

miR-641靶向TMEFF2/MEK/PI3K通路促进胰腺癌细胞干细胞特性。

Hongchao Han,Aikun Wang
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