BD Pharmingen™ Purified Rat Anti-Mouse CD31 (550274)

经验证应用

推荐实验流程

Immunocytochemistry: The MEC13.3 antibody is recommended to test for immunohistochemical staining of Zinc-fixed paraffin sections. Tissues tested were mouse spleen, lung, heart, and thymus. Immunohistochemistry of acetone-fixed frozen sections has been reported. The antibody stains endothelial cells on small and large blood vessels. The isotype control recommended for use with this antibody is purified rat IgG2a (Cat. No. 559073). For optimal indirect immunohistochemical staining, the MEC13.3 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination of biotinylated polyclonal anti-rat Igs (multiple adsorption) (Cat. No. 559286) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB Substrate Kit (Cat. No. 550880). Alternatively, the anti-rat Ig HRP Detection Kit (Cat. No. 551013) can be used to accomplish the three-step staining procedure. The clone MEC13.3 is not recommended for formalin-fixed paraffin embedded sections

制备与储存

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

荧光格式说明

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

产品提示

Since applications vary, each investigator should titrate the reagent to obtain optimal results., Source of all serum proteins is from USDA inspected abattoirs located in the United States., Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing., This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance., An isotype control should be used at the same concentration as the antibody of interest., Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

The MEC13.3 antibody specifically recognizes CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1). CD31 is a 130 kDa integral membrane protein, a member of the immunoglobulin superfamily, that mediates cell-to-cell adhesion. CD31 is expressed constitutively on the surface of adult and embryonic endothelial cells and is also expressed on many peripheral leukocytes and platelets. It has also been detected on bone marrow-derived hematopoietic stem cells and embryonic stem cells. CD31 is involved in the transendothelial emigration of neutrophils, and neutrophil PECAM-1 appears to be down-regulated after extravasation into inflamed tissues. Multiple alternatively spliced isoforms are detected during early post-implantation embryonic development; this alternative splicing is involved in the regulation of ligand specificity. CD38 and vitronectin receptor (αvβ3 integrin, CD51/CD61) are proposed to be ligands for CD31. CD31-mediated endothelial cell-cell interactions are involved in angiogenesis. The MEC13.3 mAb inhibits a variety of in vitro and in vivo functions mediated by CD31.