BD Pharmingen™ Purified Rat Anti-CD11b (550282)

经验证应用

推荐实验流程

Immunohistochemistry: The Purified Rat Anti-Mouse CD11b antibody stains mouse macrophages, granulocytes, dendritic cells, and NK cells. For optimal indirect immunohistochemical staining, investigators are encouraged to titrate this antibody (suggested starting range of a 1:10 to 1:50 dilution) and visualize via a three-step staining procedure using a combination of Biotin Goat Anti-Rat Ig (Cat. No. 559286) as the secondary antibody and Streptavidin HRP (Cat. No. 550946) together with a DAB detection system (Cat. No. 550880). Investigators should note that this antibody is not recommended for formalin-fixed paraffin embedded sections .

制备与储存

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

荧光格式说明

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

产品提示

Since applications vary, each investigator should titrate the reagent to obtain optimal results., An isotype control should be used at the same concentration as the antibody of interest., Source of all serum proteins is from USDA inspected abattoirs located in the United States., Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing., Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use., This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance., Species cross-reactivity detected in product development may not have been confirmed on every format and/or application., Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

The M1/70 monoclonal antibody specifically binds to CD11b, also known as Integrin alpha M (Itgam or αM). CD11b is a 170-kDa type 1 transmembrane glycoprotein and belongs to the Integrin alpha chain family. CD11b serves as the alpha chain of the heterodimeric Mac-1 integrin (CD11b/CD18, αMβ2), also known as complement receptor 3 (CR3). Mac-1 mediates adhesion to ICAM-1 (CD54), ICAM-2 (CD102), fibrinogen and binding to C3bi. Mac-1 is expressed at varying levels on granulocytes, macrophages, myeloid-derived dendritic cells, natural killer cells, microglia, and B-1 B lymphocytes. Mac-1 expression is rapidly upregulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. The M1/70 antibody reportedly blocks cell adherence and C3bi binding but does not block cell-mediated lysis. Cross-reaction of the M1/70 antibody with CD11b expressed on human monocytes, polymorphonuclear leukocytes, and NK cells has been reported.