Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (AB6002)

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K27me3, H3K27me, H3K27

反应种属:Human, Mouse, Bovine
抗原名称:H3C1 tri methyl K27
应用:IHC, ELISA, IF/ICC, ChIP

用户验证的应用

推荐稀释比例

  • ICC/IF5 µg/mL
  • ChIP5-10 µg for 25 µg chromatin Use Myo-D ChIP primer pair ab269261 as positive control.
  • ELISA0.025-1 µg/mL

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Mouse / IgG3
抗体类型Monoclonal
全称Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade
产品形式Liquid
储存缓冲液pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
储存条件运输条件: Blue Ice 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein A

背景信息

Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) is a mouse monoclonal antibody and is validated for use in ChIP, ELISA, ICC/IF, IHC- Wmt and WB. Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) was first used in a scientific publication in 2002 and has been cited over 982 times in peer reviewed journals. It's performance in ChIP and Western Blot in human, mouse and rat samples is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) has high sensitivity and specificity. Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) has 70 independent reviews from customers. Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002) specifically detects Histone H3 Tri Methyl-K27 (UniProt ID: P68431; Molecular weight: 16kDa) and is sold in 100 µg selling sizes. Top cited antibody for this target with >1000 citations. A highly cited Histone H3 tri methyl K27 antibody, essential for studying epigenetic modifications and gene silencing. This antibody is crucial in chromatin remodeling research, particularly in understanding the role of H3K27me3 in transcription regulation. It is widely used in studies of Polycomb repressive complexes and histone methylation. Histone H3 lysine 27 methylation (H3K27) is a key marker associated with heterochromatin formation and widely used as a marker for transcriptional repression. Want a custom formulation? This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

文献引用 (976)

Single-nucleus epigenomic profiling of the adult human central nervous system unveils epigenetic memory of developmental programs.

对成年人中枢神经系统进行单核表观基因组分析,揭示了发育程序的表观遗传记忆。

Kabbe Mukund, Agirre Eneritz, Carlström Karl E, Dumral Özge, Lor Yuk Kit, Pohl Fabio Baldivia, Ruffin Nicolas, van Bruggen David, Meijer Mandy, Seeker Luise A, Bestard-Cuche Nadine, Lederer Alex R, Zhang Jilin, Ahola Virpi, Goldman Steven A, Edström Erik, Arvidsson Lisa, Moreira Tiago Holm, Bartosovic Marek, Jagodic Maja, Williams Anna, Castelo-Branco Gonçalo

Improving erectile function in diabetic male mice by rescuing depalmitoylated FBP1 to reduce cavernosal lactate.

通过挽救去棕榈酰化的 FBP1 来降低海绵体乳酸水平,从而改善糖尿病雄性小鼠的勃起功能。

Xiao Ming, Guo Wanyang, Zeng Ruijiang, Wu Shuiqing, Jin Xin

Identification of cis-regulatory elements provides insights into tissue-specific gene regulation in the sheep genome.

顺式调控元件的鉴定有助于深入了解绵羊基因组中的组织特异性基因调控。

Xie Shangqian, Davenport Kimberly M, Salavati Mazdak, Caulton Alex, Clark Emily L, Archibald Alan L, Clarke Shannon M, Brauning Rudiger, Massa Alisha T, Mousel Michelle R, White Stephen N, Worley Kim C, Murdoch Gordon K, Becker Gabrielle, Stegmiller Morgan R, Khilji Sarem F, Shira Katie A, Olagunju Temitayo A, Hadfield Tracy, McKay Stephanie D, Rosen Benjamin D, Cockett Noelle E, Smith Timothy P L, Murdoch Brenda M

Phosphoglycerate dehydrogenase-mediated serine reprogramming aggravates macrophage hyperinflammation in murine Pseudomonas aeruginosa pneumonia.

磷酸甘油酸脱氢酶介导的丝氨酸重编程加剧了小鼠铜绿假单胞菌肺炎中的巨噬细胞过度炎症。

Chen Rong, Zeng Ran, Shi Mengmeng, Zhang Guoliang, Wang Feiyang, Yang Qingyuan, Jiang Yanshan, Zhang Yuxuan, Li Yanan, Qu Jieming

Autophagy dictates PHGDH-mediated serine metabolism in a timely manner to support oocyte development.

自噬及时调控PHGDH介导的丝氨酸代谢,以支持卵母细胞发育。

Autopolyploidization-Induced Chromatin Remodeling Regulates Leaf Size Variation in Brassica rapa.

自体多倍化诱导的染色质重塑调控甘蓝型油菜叶片大小的变化。

Dong Haoyuan, Liu Yanhong, Liu Yuanming, Xuan Shuxin, Chen Huanhuan, Wei Lai, Zhang Guibao, Pei Hongcui, Dai Zilong, Wang Yanhua, Qiao Jinzhu, Luo Shuangxia, Chen Xueping, Hong Yiguo, Zhao Jianjun, Shen Shuxing, Lu Zefu, Gu Aixia

The KDM6B/SLC10A2 Axis Suppresses MDSCs Recruitment via ERK/AP-1 Signaling in Colorectal Cancer.

KDM6B/SLC10A2轴通过ERK/AP-1信号通路抑制结直肠癌中MDSC的募集。

ERK phosphorylates HNRNPF to promote the proliferation and suppress the differentiation of embryonic stem cells.

ERK 磷酸化 HNRNPF 以促进胚胎干细胞的增殖并抑制其分化。

Duan Xiaowei, Wang Yuying, Zhang Jingai, Zhao Nannan, Hao Qianyi, Yang Xiaoqiong, Du Xiaoling, Yang Hongjie, Liang Huabin, Chen Lingyi

SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels.

哺乳期补充 SA 可通过降低 H3K27me3 水平来促进学习和记忆。

Modifying meiotic recombination by targeting chromatin regulators to crossover hotspots in Arabidopsis.

通过将染色质调节因子靶向拟南芥中的交叉热点来改变减数分裂重组。

Szymanska-Lejman Maja, Dziegielewski Wojciech, Wilhelm Anna, Hus Karolina, Ziolkowski Piotr A
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