Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (Ab6326)

BUdr, Bromodeoxyuridine

抗原名称:BrdU
应用:IHC, FCM, IF/ICC

用户验证的应用

推荐稀释比例

  • IHC-P1-3 µg/mL Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • ICC/IF1/250
  • Flow Cyt (Intra)1/25 - 1/200 Rat IgG2a, kappa monoclonal [RTK2758] (Low endotoxin, Azide Free) ( ab18450 ), is suitable for use as an isotype control with this antibody.

详细信息

宿主/亚型Rat / IgG2a
抗体类型Monoclonal
全称Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker
产品形式Liquid
储存缓冲液pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
储存条件运输条件: Blue Ice 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein G

背景信息

Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326) is a rat monoclonal antibody and is validated for use in Flow Cyt (Intra), ICC/IF and IHC-P. Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326) was first used in a scientific publication in 1985 and has been cited over 1925 times in peer reviewed journals. It's performance in IHC in mouse samples is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326) has high sensitivity and specificity. Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326) has 105 independent reviews from customers. Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326) specifically detects BrdU (UniProt ID: N/A; Molecular weight: 0.3kDa) and is sold in 100 ug and 1 mg selling sizes. Conjugation-ready, carrier free format available for antibody clone BU1/75 (ICR1) - ab264079. Antibody clone BU1/75 (ICR1) is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, Alexa Fluor ® 647, Alexa Fluor ® 594, HRP, Alexa Fluor ® 555 ( ab2274, ab2275, ab2276, ab2257, ab22124 ). Top cited antibody on the market for the proliferation marker BrdU with >2400 citations. ab6326 has over 80 5 star reviews. BrdU antibody is essential for accurately tracking cell proliferation and DNA synthesis in various research applications, including cancer and neurogenesis studies

文献引用 (2023)

PAF15-PCNA exhaustion governs the strand-specific control of DNA replication.

PAF15-PCNA 耗竭控制着 DNA 复制的链特异性调控。

Chhetri Gita, Badugu Sugith Babu, Petriman Narcis-Adrian, Petersen Mikkel Bo, Güller Aylin Seren, Fajri Nora, Coulée Manon, Pitchai Ganesha Pandian, Novotný Jan, Larsen Frederik Tibert, Møller Andreas Fønss, Ebbesen Morten Frendø, Ravnsborg Tina, Yadav Anoop Kumar, Balarasa Barath, Lunding Anita, Polasek-Sedlackova Hana, Jensen Ole N, Ravnskjaer Kim, Brewer Jonathan R, Madsen Jesper Grud Skat, Petryk Nataliya, Andersen Jens S, Somyajit Kumar

Hepatic zonation determines tumorigenic potential of mutant β-catenin.

肝脏分区决定突变β-catenin的致瘤潜力。

Raven Alexander, Gilroy Kathryn, Jin Hu, Waldron Joseph A, Leslie Holly, Munro June, Hall Holly, Ridgway Rachel A, Ford Catriona A, Gulhan Doga C, Vlahov Nikola, Mills Megan L, Hartley Andrew, Anderson Eve, Bryson Sheila, Sphyris Nathalie, Müller Miryam, May Stephanie, Cadden Barbara, Nixon Colin, Waddell Scott H, Guest Rachel, Boulter Luke, Barker Nick, Clevers Hans, Zhu Hao, Ivaska Johanna, Strathdee Douglas, Miller Crispin J, Jamieson Nigel B, Bushell Martin, Park Peter J, Bird Thomas G, Sansom Owen J

State-specific enhancer landscapes govern microglial plasticity.

特定状态的增强子图谱控制着小胶质细胞的可塑性。

Hamagami Nicole, Kapadia Dvita, Barclay Kia M, Huang Yanlong, Abduljawad Nora, Cheng Zuolin, McLaughlin Liam, Singhania Darsh, Ding Xukai, Yang Jin, Sun Zhixin, Karra Vikram, Du Siling, Bayguinov Peter, Yu Guoqiang, Li Yang E, Gabel Harrison W, Li Qingyun

FANCD2 restrains fork progression and prevents fragility at early origins upon re-replication.

FANCD2 抑制复制叉的延伸,防止在重新复制时早期复制起点出现脆弱性。

Badra-Fajardo Nibal, Karydi Elena, Bayona-Feliu Aleix, Gómez-González Belén, Preza Ourania, Arbi Marina, Kalogeropoulou Argyro, Rantala Juha K, Taraviras Stavros, Aguilera Andrés, Lygerou Zoi

Structural basis for fork reversal and RAD51 regulation by the SCF ubiquitin ligase complex of F-box helicase 1.

F-box解旋酶1的SCF泛素连接酶复合物对叉反转和RAD51调控的结构基础。

Greer Briana H, Mendia-Garcia Javier, Mullins Elwood A, Peacock Emma M, Haigh Sander K, Schiltz Carl J, Aicart-Ramos Clara, Tsai Miaw-Sheue, Cortez David, Moreno-Herrero Fernando, Eichman Brandt F

Mechanistic insights into PCBP1-driven unfolding of selected i-motif DNA at G(1)/S checkpoint.

从机制上深入了解 PCBP1 驱动的 G(1)/S 检查点特定 i-motif DNA 的展开。

Sengupta Pallabi, Gillet Natacha, Obi Ikenna, Sabouri Nasim

The nuclease EXO1 promotes genomic instability by degrading nascent DNA in BRCA-proficient cells.

核酸酶 EXO1 通过降解 BRCA 功能正常细胞中的新生 DNA 来促进基因组不稳定。

Nusawardhana Alexandra, Nicolae Claudia M, Moldovan George-Lucian

Anti-CSF-1R therapy with combined immuno-chemotherapy coordinate an adaptive immune response to eliminate macrophage enriched triple negative breast cancers.

抗 CSF-1R 疗法与免疫化疗相结合,可协调适应性免疫反应,以消除富含巨噬细胞的三阴性乳腺癌。

Pedroza Diego A, Yuan Xueying, Liu Fengshuo, Chan Hilda L, Zhang Christina, Bowie William, Smith Alex J, Calderon Sebastian J, Lieu Nadia, Wu Weiguo, Porter Paul, Sarkar Poonam, Zhao Na, Oehler Constanze V, Peller Ondrej, Gaber M Waleed, Zhu Qian, Perou Charles M, Zhang Xiang H-F, Rosen Jeffrey M

NF-κB activation in astrocytes impairs wound healing after traumatic brain injury in male mice.

星形胶质细胞中 NF-κB 的激活会损害雄性小鼠脑外伤后的伤口愈合。

Hein Tabea M, Nespoli Ester, Hakani Marsela, Wendt Heinrich, May Stephanie Nadine, Jorzik Jasmin, Yagdiran Duygu, Schlett Judith S, Tsesmelis Konstantinos, Tsesmelis Miltiadis, Prex Vivien, Mettang Melanie, Abaei Alireza, Rasche Volker, Lattke Michael, Oswald Franz, Huber-Lang Markus, Dimou Leda, Wirth Thomas, Baumann Bernd

Dynamic allele usage of X-linked genes ameliorates neurodevelopmental disease phenotypes in brain organoids.

X连锁基因的动态等位基因使用可改善脑类器官中的神经发育疾病表型。

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